Asthagiri Cell Engineering Laboratory

Papers

Luo, C. Y., R. J. Natividad, M. L. Lalli, and A. R. Asthagiri. 2020. Multivariate relationships among nucleus and Golgi properties during fibrillar migration are robust to and unchanged by epithelial-to-mesenchymal transition. PLoS One. 15:e0239188. Link.

Natividad, R. J., M. L. Lalli, S. K. Muthuswamy, and A. R. Asthagiri. 2018. Golgi Stabilization, Not Its Front-Rear Bias, Is Associated with EMT-Enhanced Fibrillar Migration. Biophys J. 115:2067-2077.

Lalli, M. L., B. Wojeski, and A. R. Asthagiri. 2017. Label-Free Automated Cell Tracking: Analysis of the Role of E-cadherin Expression in Collective Electrotaxis. Cellular and Molecular Bioengineering. 10:89-101.

Milano, D. F., R. J. Natividad, Y. Saito, C. Y. Luo, S. K. Muthuswamy, and A. R. Asthagiri. 2016. Positive Quantitative Relationship between EMT and Contact-Initiated Sliding on Fiber-like Tracks. Biophysical Journal. 111:1569-1574. Link.

Milano, D. F., N. A. Ngai, S. K. Muthuswamy, and A. R. Asthagiri. 2016. Regulators of Metastasis Modulate the Migratory Response to Cell Contact under Spatial Confinement. Biophys J. 110:1886-1895.

Lalli, M. L., and A. R. Asthagiri. 2015. Collective migration exhibits greater sensitivity but slower dynamics of alignment to applied electric fields. Cell Mol Bioeng. 8:247-257.

Walsh, D. I., M. L. Lalli, J. M. Kassas, A. R. Asthagiri, and S. K. Murthy. 2015. Cell chemotaxis on paper for diagnostics. Anal Chem. 87:5505-5510.

Viswanathan, K., and A. R. Asthagiri. 2014. Micropatterned polyacrylamide gel for investigating the effect of compliant, fiber-like topology on cell behaviors. 2014 40th Annual Northeast Bioengineering Conference.

Lalli, M. L., and A. R. Asthagiri. 2014. Investigating electrotaxis of the non-transformed MCF-10A mammary epithelial cell line. 2014 40th Annual Northeast Bioengineering Conference.

Bouffard, J., A. R. Asthagiri, and E. J. Cram. 2014. Developing a biosensor-labeled model tissue for in vivo studies of cellular and molecular mechanotransduction. 2014 40th Annual Northeast Bioengineering Conference.

Milano, D. F., and A. R. Asthagiri. 2014. An ex vivo platform for quantitatively analyzing pairwise interactions between cancer cells. 2014 40th Annual Northeast Bioengineering Conference.

Natividad, R. J., and A. R. Asthagiri. 2014. A micropatterned microfluidic device for engineering soluble and tethered environmental cues in cell culture. 2014 40th Annual Northeast Bioengineering Conference.

Minor, P. J., T. F. He, C. H. Sohn, A. R. Asthagiri, and P. W. Sternberg. 2013. FGF signaling regulates Wnt ligand expression to control vulval cell lineage polarity in C. elegans. Development. 140:3882-3891.

Blagovic, K., E. S. Gong, D. F. Milano, R. J. Natividad, and A. R. Asthagiri. 2013. Engineering cell-cell signaling. Curr Opin Biotechnol. 24:940-947.

Notbohm, J., J.-H. Kim, C. Franck, S. Maskarinec, D. Tirrell, A. Asthagiri, and G. Ravichandran. 2012. Three-dimensional Traction Force Microscopy for Studying Cellular Interactions with Biomaterials. Procedia IUTAM. 4:144-150.

Notbohm, J., J. H. Kim, A. R. Asthagiri, and G. Ravichandran. 2012. Three-dimensional analysis of the effect of epidermal growth factor on cell-cell adhesion in epithelial cell clusters. Biophys J. 102:1323-1330.

Pope, M. D., and A. R. Asthagiri. 2012. Short-lived, transitory cell-cell interactions foster migration-dependent aggregation. PLoS One. 7:e43237.

Kushiro, K., and A. R. Asthagiri. 2012. Modular design of micropattern geometry achieves combinatorial enhancements in cell motility. Langmuir. 28:4357-4362.

Kim, J. H., and A. R. Asthagiri. 2011. Matrix stiffening sensitizes epithelial cells to EGF and enables the loss of contact inhibition of proliferation. J Cell Sci. 124:1280-1287.

Notbohm, J., J. H. Kim, A. Asthagiri, and G. Ravichandran. 2011. Application of 3D Traction Force Microscopy to Mechanotransduction of Cell Clusters. Applied Mechanics and Materials. 70:21-27.

Kushiro, K., S. Chang, and A. R. Asthagiri. 2010. Reprogramming directional cell motility by tuning micropattern features and cellular signals. Adv Mater. 22:4516-4519.

Kim, J. H., L. J. Dooling, and A. R. Asthagiri. 2010. Intercellular mechanotransduction during multicellular morphodynamics. J R Soc Interface. 7 Suppl 3:S341-50.

Kim, J. H., K. Kushiro, N. A. Graham, and A. R. Asthagiri. 2009. Tunable interplay between epidermal growth factor and cell-cell contact governs the spatial dynamics of epithelial growth. Proc Natl Acad Sci U S A. 106:11149-11153.

Giurumescu, C. A., and A. R. Asthagiri. 2009. Systems Approaches to Developmental Patterning. In Systems Biomedicine: Concepts and Perspectives, Liu, E. T., and D. A. Lauffenburger, editors. 329-350.

Kim, J.-H., and A. R. Asthagiri. 2009. Quantitative Immunofluorescence for Measuring Spatial Compartmentation of Covalently Modified Signaling Proteins. In Systems Analysis of Biological Networks, Jayaraman, A., and J. Hahn, editors. Artech House, 1-10.

Chapman, S. A., and A. R. Asthagiri. 2009. Quantitative effect of scaffold abundance on signal propagation. Mol Syst Biol. 5:313.

Giurumescu, C. A., P. W. Sternberg, and A. R. Asthagiri. 2009. Predicting phenotypic diversity and the underlying quantitative molecular transitions. PLoS Comput Biol. 5:e1000354.

Giurumescu, C. A., and A. R. Asthagiri. 2008. Signal processing during developmental multicellular patterning. Biotechnol Prog. 24:80-88.

Pope, M. D., N. A. Graham, B. K. Huang, and A. R. Asthagiri. 2008. Automated quantitative analysis of epithelial cell scatter. Cell Adh Migr. 2:110-116.

Galownia, N. C., K. Kushiro, Y. Gong, and A. R. Asthagiri. 2007. Selective desensitization of growth factor signaling by cell adhesion to fibronectin. J Biol Chem. 282:21758-21766.

Graham, N. A., M. D. Pope, T. Rimchala, B. K. Huang, and A. R. Asthagiri. 2007. A microtiter assay for quantifying protein-protein interactions associated with cell-cell adhesion. J Biomol Screen. 12:683-693.

Giurumescu, C. A., P. W. Sternberg, and A. R. Asthagiri. 2006. Intercellular coupling amplifies fate segregation during Caenorhabditis elegans vulval development. Proc Natl Acad Sci U S A. 103:1331-1336.

Richman, G. P., D. A. Tirrell, and A. R. Asthagiri. 2005. Quantitatively distinct requirements for signaling-competent cell spreading on engineered versus natural adhesion ligands. J Control Release. 101:3-12.

Chapman, S., and A. R. Asthagiri. 2004. Resistance to signal activation governs design features of the MAP kinase signaling module. Biotechnol Bioeng. 85:311-322.

Graham, N. A., and A. R. Asthagiri. 2004. Epidermal growth factor-mediated T-cell factor/lymphoid enhancer factor transcriptional activity is essential but not sufficient for cell cycle progression in nontransformed mammary epithelial cells. J Biol Chem. 279:23517-23524.

Asthagiri, A. R., and D. A. Lauffenburger. 2001. A computational study of feedback effects on signal dynamics in a mitogen-activated protein kinase (MAPK) pathway model. Biotechnol Prog. 17:227-239.

Asthagiri, A. R., C. A. Reinhart, A. F. Horwitz, and D. A. Lauffenburger. 2000. The role of transient ERK2 signals in fibronectin- and insulin-mediated DNA synthesis. J Cell Sci. 113 Pt 24:4499-4510.

Asthagiri, A. R., and D. A. Lauffenburger. 2000. Bioengineering models of cell signaling. In 2, editors. 31-53.

Asthagiri, A. R., C. M. Nelson, A. F. Horwitz, and D. A. Lauffenburger. 1999. Quantitative relationship among integrin-ligand binding, adhesion, and signaling via focal adhesion kinase and extracellular signal-regulated kinase 2. J Biol Chem. 274:27119-27127.

Asthagiri, A. R., A. F. Horwitz, and D. A. Lauffenburger. 1999. A rapid and sensitive quantitative kinase activity assay using a convenient 96-well format. Anal Biochem. 269:342-347.